Establishment of the differentiation method to spermatogenesis by the xenogeneic transplantation of spermatogonia stem cells and the in vitro culture

2015 Fiscal Year Final Research Report

• Project/Area Number: 24590243
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tokyo Medical University
• Principal Investigator: Qu Ning 東京医科大学, 医学部, 講師 (70527952)
• Co-Investigator(Kenkyū-buntansha): ITOH Masahiro 東京医科大学, 医学部, 教授 (00232471) ; YOSHIMOTO Takayuki 東京医科大学, 医学部, 教授 (80202406)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 精子形成 / 精子幹細胞 / 異種移植 / 免疫寛容 / マウス

Outline of Final Research Achievements

There have been increasing reports on xenogeneic transplantation of mammalian male spermatogonial stem cells (SSCs). However, in most previous studies on xenogeneic SSCs transplantation, to avoid immunologic rejection, immunodeficient or immunosuppressed mice were used as the recipients, which were pre-treated with busulfan (Myleran, 1,4-butanediol methanesulfonate) for depletion of endogenous germ cells before the SSCs transplantation. This study demonstrated that in the testes of busulfan-treated immune-competent mice, the increase of TNF-a in the seminiferous tubule and the infiltration of the macrophage were seen in the interstitium, and the increase of apoptotic cytokine such as Fas,FasL,caspase8 and the decrease of caspase9 were detected. Furthermore, it was revealed that immune-suppressive cytokines and M2 macrophage in the testis are related with testicular immune privileged circumstances.

Free Research Field

生殖免疫学