Studies of complement-related mechanisms and possibilities of anti-complement therapy for peritoneal injuries under PD therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Nagoya University
• Principal Investigator: MASASHI Mizuno 名古屋大学, 医学(系)研究科(研究院), 寄附講座准教授 (20303638)
• Co-Investigator(Kenkyū-buntansha): ITO Yasuhiko 名古屋大学, 大学院医学系研究科, 寄附講座教授 (60402632) ; SUZUKI Yasuhiro 名古屋大学, 大学院医学系研究科, 寄附講座助教 (20584676) ; MATSUO Seiichi 名古屋大学, 大学院医学系研究科, 教授 (70190410)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 人工透析学 / 腹膜透析 / 補体活性化 / 補体制御 / 腹膜障害

Outline of Final Research Achievements

We clarified that plenty of CReg, CD46, CD55 and CD59 were expressed in mesothelial cell of human peritoneum. When we investigated whether performance of PD therapy influenced the complement system in PD patients, we got correlation between levels of sC5b-9 in PD fluid and D/P creatinine and reverse-correlation between D/P creatinine and expression of CD55 in peritoneal mesothelium in the PD patients. Between serum levels and PD fluids, C4 was well correlated but not C3. Our results suggested that PD therapy might change CD55 expression of mesothelium and influence D/P creatinine in the PD patient dependent on activation of the alternative pathway presumably.
Using an acute peritonitis model on rat, we showed the supportive data of the possibilities of anti-complement therapies because suppression of C5a could improve the acute peritoneal injuries.

Free Research Field

内科学