2014 Fiscal Year Final Research Report
Autoantibodies to cofilin-1 in patients with Behcet's Disease
| Project/Area Number |
24591458
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
SEIDO Ooka 聖マリアンナ医科大学, 医学部, 講師 (30367370)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 自己抗体 / コフィリン |
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| Outline of Final Research Achievements |
To elucidate the autoimmunity in Beh?et's Disease (BD), we surveyed autoantigens (autoAgs) in patients with BD and investigated prevalence and clinical significance of the identified autoantibodies (autoAbs).
We prepared whole cofilin-1 and three fragments as fusion protein with maltose binding protein. The autoAbs to cofilin-1, were investigated by Enzyme-Linked ImmunoSorbent Assay using fusion protein of cofilin-1 in 60 patients with BD. The autoAbs to cofilin-1 were detected by WB in 11 (18.3%) of the 60 patients with BD. Multiple epitopes on cofilin-1 were recognized by 45.5% of the anti-cofilin-1 positive serum samples. The autoAbs to cofilin-1 are probably produced by the antigen-driven mechanism. Clinically, the existence of the autoAbs to cofilin-1 correlated with the erythrocyte sedimentation rate and production of interferon alpha.
Our data indicates that the generation of autoAbs to cofilin-1 may reflect common immunological disorders in BD.
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| Free Research Field |
自己免疫疾患
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