Investigation of pediatric mucosal immune system and pathogenesis of pediatric onset gastrointestinal diseases.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591585
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Juntendo University
• Principal Investigator: OHTSUKA Yoshikazu 順天堂大学, 医学部, 客員准教授 (90338335)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Toshiaki 順天堂大学, 医学部, 教授 (30260889) ; SUZUKI Ryuyo 順天堂大学, 医学部, 准教授 (70420859)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 食物アレルギー / 新生児・乳児消化管アレルギー / 炎症性腸疾患 / クローン病 / 潰瘍性大腸炎 / リンパ濾胞 / CXCL13

Outline of Final Research Achievements

To investigate the pathogenesis of pediatric onset gastrointestinal diseases, such as food protein-induced proctocolitis (FPIP), neonatal transient eosinophilic colitis (NTEC), Crohn’s disease (CD), and ulcerative colitis (UC), we examined the mucosal expression of inflammatory signals using microarray, RT-PCR, and immunohistochemical analysis. Enhanced expression of IL-6, CCL11, and CXCL13 was confirmed in both NTEC and FPIP. Mucosal infiltration of CD3- and IgA- but not IgE-positive cells was confirmed in both. Studies with CD and UC revealed that the expression of CXCL9, 10, 11 and MMP-1, -3, -7, -10 was significantly enhanced in active phase of pediatric CD and UC, respectively. Meanwhile, enhanced expression of CXCL13 was confirmed among these diseases suggested that IgA synthesis targeting multiple antigens is an important task for the mucosal immune systems during infancy since CXCL13 is a chemokine related to lymphoid follicle formation leading to IgA synthesis.

Free Research Field

小児消化器病学