The effects of glucocorticoid on the expression of caveolin-1 in skin-related cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591654
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kagawa University
• Principal Investigator: KUBOTA YASUO 香川大学, 医学部, 教授 (10126047)
• Co-Investigator(Kenkyū-buntansha): IGARASI Jyunsuke 香川大学, 医学部, 准教授 (20346638) ; KOSAKA Hiroaki 香川大学, 医学部, 教授 (60158897)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: カベオリン / 皮膚 / ステロイド / 血管内皮細胞 / VEGF / シグナル伝達

Outline of Final Research Achievements

We examined the effects of glucocorticoid agents that are frequently used in clinic at dermatology, by using cultured skin-related cells as a model. When vascular endothelial cells derived from humans or cows were treated with glucocorticoids, the expression levels of a key signal regulating molecule termed caveolin-1 were markedly up-regulated at the levels of protein and mRNA. Glucocorticoids were without effect for keratinocytes and fibroblasts, two other major skin component cells. We then explored the effects of glucocorticoids on signaling responses of endothelial cells for stimulation with vascular endothelial growth factor. Markedly attenuated responses were obtained in glucocorticoids-treated cells, which were counteracted by gene knockdown of caveolin-1. Similar observations were obtained in living animals. Thus, our study uncovered previously unidentified effects of glucocorticoids on dermatological cells, in which induction of caveolin-1 plays a key role.

Free Research Field

皮膚の炎症と血管新生