Treatment strategy of pancreatic cancer based on genome-wide profile

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: HIRONO Seiko 和歌山県立医科大学, 医学部, 講師 (60468288)
• Co-Investigator(Kenkyū-buntansha): YAMAUE Hiroki 和歌山県立医科大学, 医学部, 教授 (20191190) ; TANI Mesuji 和歌山県立医科大学, 医学部, 准教授 (60236677) ; KAWAI Manabu 和歌山県立医科大学, 医学部, 講師 (40398459) ; OKADA Ken-ichi 和歌山県立医科大学, 医学部, 助教 (50407988) ; MIYAZAWA Motoki 和歌山県立医科大学, 医学部, 学内助教 (90549734)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 網羅的遺伝子解析 / 包括的DNA1次構造異常解析 / 膵癌

Outline of Final Research Achievements

We identified specific copy number variants and heterogenous deletion for pancreatic cancer by GeneChip Mapping array 100K and expression profile by GeneChip Human Genome U133 plus 2.0 array, using DNA and RNA extracted from harvest the pancreatic cancer cells by microdissection. We identified 6 regions, including Ch 9p21.3, Ch 18q21.1, of homozygous deletion, and many regions of heterogenous deletion. The RNA expression of CDKN2A gene, which is located in Ch 9p21.3, and SMAD4, which is located in Ch 18q21.1, were none in homozygous deletion samples. However the RNA expression of these genes were various in heterogenous deletion samples. We also identified some specific amplification regions and we found that the RNA expression of the genes which is located in these regions increased in amplification samples, compared to other samples.

Free Research Field

膵臓癌