The effect of autophagy activator to neural cells under hypoxic condition

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kagoshima University
• Principal Investigator: TANABE Fumito 鹿児島大学, 医歯(薬)学総合研究科, 助教 (90619199)
• Co-Investigator(Kenkyū-buntansha): KOMIYA Setsuro 鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371) ; SETOGUCHI Takao 鹿児島大学, 大学院医歯学総合研究科, 特任准教授 (40423727)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 神経幹細胞 / iPS細胞 / オートファジー / 低酸素

Outline of Final Research Achievements

We used the neural stem cell cell line AF22 which is derived by human iPS cell.The expression of LC3-II, which is a marker of autophagy, was upregulated in 1%O2 condition compared to 20% O2condition. LiCl which is an autophagy activator increased the proliferation of AF22. In addition, rapamycin which is an another autophagy activator increased the proliferation of AF22 under 1% O2 and 20% O2 conditions. Furthermore, differentiation capacity of AF22 was not chaged under 1% O2 condition.

Free Research Field

脊髄損傷