Analysis of appropriate cell source for efficient annulus fibrosis regeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokai University
• Principal Investigator: SAKAI Daisuke 東海大学, 医学部, 准教授 (10408007)
• Co-Investigator(Kenkyū-buntansha): MOCHIDA Joji 東海大学, 医学部, 教授 (30129697) ; HIYAMA Akihiko 東海大学, 医学部, 講師 (00514382) ; NAKAI Tomoko 東海大学, 医学部, 特定研究員 (20624589)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 椎間板 / 再生医療 / 幹細胞 / 線維輪

Outline of Final Research Achievements

Altered function of the intervertebral disc leads to various degenerative spine disorders. We established an isolation method for retrieving purely annulus fibrosus cells from intervertebral disc of C57BL/6 mouse. We then tested whether CD146, a marker for mesenchymal stem cells in various organs, relates to proliferation and differentiation of annulus fibrosis cell. We isolated AF tissue from mouse and cultured isolated cells to analyze their cell surface markers and multi-potency. Result showed that mouse annulus fibrosus cells differentiated to three mesenchymal lineages. Furthermore, CD 146 positive annulus fibrosus cells became proliferative with hypoxia, TGF-beta 1 or R3-IGF1 stimuli suggesting its role as a useful differentiation marker for annulus fibrosus regeneration.

Free Research Field

整形外科学