Role of GLP-1 in volatile anesthetic induced cardiac protection

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: The University of Tokushima
• Principal Investigator: OSHITA Shuzo 徳島大学, ヘルスバイオサイエンス研究部, 非常勤講師 (60144945)
• Co-Investigator(Kenkyū-buntansha): TANAKA Katsuya 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (30263841) ; TSUTSUMI Yasuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (90523499)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: GLP-1受容体 / 虚血再潅流障害 / 吸入麻酔薬

Outline of Final Research Achievements

Exendin-4, GLP-1 receptor agonist, protected cardiac myocytes (CM) from ischemia-reperfusion. Additionally, myocardial infarct size was smaller in the isoflurane- [1.0 minimum alveolar concentration (MAC)] or exendin-4- (30 ng/kg i.v.) treated groups than the controls. Infarct size was not affected by isoflurane at 0.5 MAC or 3 ng/kg i.v. exendin-4, but the combination of these treatments reduced infarct size.
Immunofluorescence analysis of the expression and colocalization of caveolin-3 (Cav-3) and GLP-1R in cardiac myocytes. Fluorescent secondary antibodies were used to determine Cav-3 and GLP-1R localization, and strong colocalization were observed on the cell surface membrane. Additionally, these preconditioning significantly attenuated the calcium-induced swelling compared with the control.

Free Research Field

麻酔科学