2014 Fiscal Year Final Research Report
Analysis of endometrial cancer stem cells
| Project/Area Number |
24592508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KYO Satoru 島根大学, 医学部, 教授 (50272969)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 子宮内膜癌 / 癌幹細胞 |
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| Outline of Final Research Achievements |
Previous our study demonstrated that CD133+ enodometrial cancer cells showed tumorigenic and self-renewal ability, suggesting that CD133 was a potential CSC marker in endometrial cancer. In this study, we focused on multilineage differentiation and investigated an ability of endothelial differentiation in endometrial CSCs. Immunohistochemistry of subcutaneous xenografts showed that human endometrial tumors contained human vessels labeled by human-specific anti-CD31 antibodies. Endothelial tube formation assay (in vitro angiogenesis) revealed that isolated CD133+ endometrial cancer cells could show tube formation while CD133- cells could not. CD133+ cells expressed VEGFR-1 higher than CD133- cells. Furthermore, in vitro angiogenesis assay demonstrated hypoxic condition could promote and keep tube formation in CD133+ cells, but not CD133- cells.
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| Free Research Field |
婦人科腫瘍学
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