An effective screening for proliferative vitreoretinopathy-associated genes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592687
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Fukuoka University
• Principal Investigator: SASSA, Yukio 福岡大学, 医学部, 講師 (80580315)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Shigeo 九州大学, 医学部, 講師 (50363370) ; ISHIBASHI Tatsuro 九州大学, 医学部, 教授 (30150428) ; FUKUSHIMA Syuuichiro 大阪大学, 基礎工学部, 助教 (40362644) ; YASUI Takeshi 徳島大学, 工学部, 教授 (70314408) ; KONO Tosihiro 福岡大学, 医学部, 教授 (40117106)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 眼増殖性疾患 / 網膜色素上皮細胞 / SHG光 / コラーゲン / リモデリング

Outline of Final Research Achievements

We recently have revealed the ocular proliferation-associated genes by microarray analysis of intraocular proliferative tissues. We have previously reported a new realtime collagen-remodeling models using the SHG microscope.　SHG microscope is based on a nonlinear optical effect known as second-harmonic generation and can image collagen structures without staining. In this project, we use the multi-well culture slides(μ-Slide Angiogenesis, ibidi) to evaluate the effect of multi proteins on collagen remodeling at once. We evaluated the effect of occular proliferation-associated proteins including periostin and tenacin C on the collagen remodeling. We also revealed that periostin and tenacin C promoted the retinal cell(retinal endothelial cell and retinal pigment epithelial cell) migration, proliferation and tube-formation.

Free Research Field

眼科学