2013 Fiscal Year Final Research Report
Clarify the acquisition and retention mechanisms of epigenetic information by use of beating cardiomyocytes
| Project/Area Number |
24650253
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hosei University (2013)
Tokyo Medical and Dental University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 心筋細胞 / 細胞外電位計測 / 拍動周期 / 後天的情報 |
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| Research Abstract |
To quantify the epigenetic information accumulated in cells, I observed the relaxation process after adding perturbations to beating cardiomyocytes for model cells. First, I developed the system of simultaneous measurement of beating rate and field potential in beating cardiomyocytes for measurement of correlation between beating rate and active state of ion channels. Next, I measured the active state of ion channels and the beating rate on cardiomyocytes adding to perturbation for electrical stimulations, drug effects, and temperature changes. As a result, active state of ion channels was logarithmically changed by the beating rate fixed with electrical stimulation, and at the stopping of electrical stimulation it was logarithmically reverted to the active state before adding perturbation. Finally, these results were clarified a part of acquisition and retention mechanism of epigenetic information by analysis of relaxation process after perturbations.
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Research Products
(30 results)
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[Presentation] Toward quasi-in vivo from in vitro assay (I). On-chip cardiomyocyte network screening assay for predictive cardiotoxicity, No.0882012
Author(s)
K. Yasuda, F. Nomura, T. Hamada, T. Kaneko, H. Takamori, Y. Abe, T. Sakakura, K. Takasuna, A. Sanbuissho
Organizer
Safety Pharmacology Society 12th Annual Meeting
Place of Presentation
Phoenix, USA
Year and Date
20121001-04
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[Presentation] Toward Quasi-In Vivo from In Vitro assay (III). Evaluation of Temporal Field Potential Duration Fluctuation and Spatial Conduction Velocity Fluctuation of Cardiomyocyte Network for In Vitro Predictive Cardiotoxicity Measurement, No.0902012
Author(s)
T. Hamada, F. Nomura, T. Kaneko, H. Takamori, Y. Abe, T. Sakakura, K. Takasuna, A. Sanbuissho, K. Yasuda
Organizer
Safety Pharmacology Society 12th Annual Meeting
Place of Presentation
Phoenix, USA
Year and Date
20121001-04
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