2013 Fiscal Year Final Research Report
Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles
| Project/Area Number |
24650294
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical systems
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| Research Institution | Kanazawa University |
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Principal Investigator |
SHIMIZU Nobuaki 金沢大学, 環日本海域環境研究センター, 教授 (50019634)
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Kazuaki 金沢大学, 環日本海域環境研究センター, 准教授 (10379125)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 超音波 / 二酸化チタン / ナノ粒子 / OHラジカル / がん幹細胞 |
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| Research Abstract |
In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm2, 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24 h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.
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[Journal Article] Evaluation of extra- and intracellular OH radical generation, cancer cell injury, and apoptosis induced by a non-thermal atmospheric pressure plasma jet2013
Author(s)
Kazuaki Ninomiya, Tatsuo Ishijima, Masatoshi Imamura, Takayuki Yamahara, Hiroshi Enomoto, Kenji Takahashi, Yasunori Tanaka, Yoshihiko Uesugi, Nobuaki Shimizu
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Journal Title
J. Phys. D : Appl. Phys.
Volume: 46(42)
Pages: 425401
DOI
Peer Reviewed
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