Analysis of protein folding and conformational change based on the characterization of the transition state

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657103
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: IMAMOTO Yasushi 京都大学, 理学(系)研究科(研究院), 准教授 (80263200)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 光生物学 / 構造変化 / 異性化 / 折りたたみ / 中間体 / 吸収スペクトル / 光センサー

Outline of Final Research Achievements

Photoactivation of photoactive yellow protein yields the physiologically active state called PYPM. In PYPM, the structure is partially unfolded, and photocycle of PYP is a good model to study the protein folding. Here we analyzed the photocycle of PYP based on the characterization of the transition state between the intermediates.

Free Research Field

生物物理学