Development of a new metalloprotein based on the structure and function of metalloenzymes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658287
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: MASUDA Taro 京都大学, (連合)農学研究科(研究院), 助教 (40395653)
• Co-Investigator(Kenkyū-buntansha): MIKAMI Bunzo 京都大学, 大学院農学研究科, 教授 (40135611)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 蛋白質 / 立体構造 / 金属 / リガンド

Outline of Final Research Achievements

To establish the relationship between a protein and its metal ligand, we divided the research to the following two parts. 1. X-ray crystallographic studies on novel metal binding proteins, and 2. Designing a new metal binding protein based on the structure and function of known metalloproteins. As the results of the first approach, we determined the crystal structures of a copper binding prophenoloxidase from crustacean and a calcium accumulating class III chitinase from pomegranate. Further, we constructed a novel nickel binding protein based on the iron storage protein ferritin.

Free Research Field

食品生物化学