The role of innate immunity in Dilated Cardiomyopathy

2012 Fiscal Year Final Research Report

• Project/Area Number: 24659145
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: MIYAKE Kensuke 東京大学, 医科学研究所, 教授 (60229812)
• Co-Investigator(Renkei-kenkyūsha): SHIBATA Takuma 東京大学, 医科学研究所, 特任助教 (30554505)
• Project Period (FY): 2012
• Keywords: 拡張型心筋症 / TLR

Research Abstract

We constructed Epithelial Membrane Protein 3 Transgenic mice (Emp3 Tg) which spontaneously suffered from DCM like heart symptoms. Emp3 Tg mice with CAG-Cre gene died within 6 months and most of them showed dilated heart atria and ventricles. However, deleting CAG-Cre gene from Emp3 Tg mice relieved them from DCM like symptoms. Considering that CAG-Cre did not show DCM like symptoms at all, Emp3 gene may exacerbate unknown abnormality in heart induced by Cre proteinoverexpression.

Research Products

• [Journal Article] Structural Reorganization of the Toll-Like Receptor 8 Dimer Induced by Agonistic Ligands (2013)
  • Author(s): Tanji H, Ohto U, Shibata T, Miyake K, Shimizu T
  • Journal Title: Science Volume: 339巻 Pages: 1426-1429
  • DOI: DOI:10.1126/science.1229159
  • Peer Reviewed
• [Journal Article] Essential role for Toll-like receptor 7 (TLR7)-unique cysteines in an intramolecular disulfide bond, proteolytic cleavage and RNA sensing. (2013)
  • Author(s): Kanno A, Yamamoto C, Onji M, Fukui R, Saitoh SI, Motoi Y, Shibata T, Matsumoto F, Muta T, Miyake K.
  • Journal Title: International Immunology
  • DOI: DOI:10.1093/intimm/dxt007
  • Peer Reviewed
• [Journal Article] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Shibata T, Takemura N, Motoi Y, Goto Y, Karuppuchamy T, Izawa K, Li X, Akashi-Takamura S, Tanimura N, Kunisawa J, Kiyono H, Akira S, Kitamura T, Kitaura J, Uematsu S, Miyake K.
  • Journal Title: International Immunology Volume: 24巻,10号 Pages: 613-23
  • DOI: DOI:10.1093/intimm/dxs068
  • Peer Reviewed
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Shibata Takuma, Takemura Naoki, Motoi Yuji, Goto Yoshiyuki, Uematsu Satoshi, Miyake Kensuke.
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸ポー トピアホテル、兵庫県
  • Year and Date: 2012-12-06
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Takuma Shibata, Naoki Takemura, Yuji Motoi, Satoshi Uematsu, Kensuke Miyake.
  • Organizer: The 12th Biennial International Endotoxin & Innate Immunity Society(IEIIS) meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-10-23
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/kanseniden/index.html