2013 Fiscal Year Final Research Report
Screening of novel anti-citrullinated protein antibodies for rheumatoid arrhtiris by protein array
| Project/Area Number |
24659274
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
OHMURA Koichiro 京都大学, 医学(系)研究科(研究院), 講師 (40432372)
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| Co-Investigator(Renkei-kenkyūsha) |
SAWASAKI Tatsuya 愛媛大学, 無細胞生命科学工学研究センター, 准教授 (50314969)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 免疫血清学 / 抗CCP抗体 / ACPA / 関節リウマチ / バイオマーカー / 抗CCP抗体陰性RA / AlphaScreen法 / 蛋白アレイ |
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| Research Abstract |
Anti-cyclic citrullinated peptide (CCP) antibody is a specific autoantibody against rheumatoid arthritis (RA). However, it becomes positive in only about 50% of early RA patients, and sometimes it is difficult to correctly diagnose RA in the early phase. Therefore, the diagnostic biomarkers for anti-CCP antibody-negative RA have been required. We screened the anti-CCP antibody-negative RA sera for novel anti-citrullinated protein antibodies (ACPA) using AlphaScreen method. We found 14 novel anti-citrullinated protein antibodies from over 2,000 citrullinated proteins, out of which 2 were assured by Western blotting and ELISA.
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