Technical development of microscopic replantation of animal leg frozen with CAS freezer.

2012 Fiscal Year Final Research Report

• Project/Area Number: 24659787
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Dokkyo Medical University
• Principal Investigator: SANO Kazufumi 獨協医科大学, 医学部, 准教授 (40287769)
• Co-Investigator(Kenkyū-buntansha): OZEKI Satoru 獨協医科大学, 医学部, 教授 (50223821) ; KIMURA Kazumasa 獨協医科大学, 医学部, 助教 (00296146) ; HASHIMOTO Tomohisa 獨協医科大学, 医学部, 助教 (70364635) ; MASUDA Yoko 獨協医科大学, 医学部, 助教 (60534112)
• Project Period (FY): 2012
• Keywords: マイクロサージェリー学 / 凍結医学

Research Abstract

Usual freezing process causes completely cell death in the vital tissue. Cells Alive System (CAS) is a particular freezing technique which restrains cell death during freezing at minimum. Purpose of this research was to develop replantation technique of amputated, CAS frozen, and thawed animal leg. As results, although replanted leg resulted in complete necrosis eventually, very useful findings for the future were achieved. That is, progress of performance of CAS freezer as to produce quicker and more uniform freezing, and adjustment and preparation of cryopreservation solution would lead to future success.