Semi-synthesis of receptor tyrosine kinase and its structure and function analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 24687013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Sato Takeshi 大阪大学, たんぱく質研究所, 講師 (90403013)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 膜タンパク質 / 半合成 / 膜貫通―膜近傍部位

Outline of Final Research Achievements

Receptor tyrsine kinase is a transmembrane receptor which initiates a signaling for cell growth and differentiation. Dysfunction of the receptor can be a cause of cancer. In thie project, focuing on the transmembrane-juxtamembrane region of the receptor, which has been one of the most difficult portions in the field of structural biology, the structural analysis was performed. Results provided a new concept in the activation mechanism of receptor tyrosine kinase, which is the release of the intracellular juxtamembrane region form the inner leaflet of the plasma membrane. Also, the strategy of its semi-synthesis is developed. Semi-synthesis chemistry to couple expressed protein and chemically synthesized protein fragments. A portion of the receptor, from the extracellular region to the transmembrane-itracellular juxtamembrane region, was successfully synthesized.

Free Research Field

構造生物化学