2013 Fiscal Year Final Research Report
Functional analysis of CTCF for generating neuronal diversity and neuronal networks
| Project/Area Number |
24700410
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | CTCF / Neuron / diversity |
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| Research Abstract |
I generated postmitotic neurons specific CTCF conditional knockout mice and investigated the role of the brain and the regulation of clustered Pcdh genes. The CTCF-cKO mice exhibited growth retardation, abnormal behavior, and died within one month. Gene expression profile exhibited significant downregulation of clustered Protocadherin genes. I examined structure of somatosensory cortex and found that barrel structure was disappeared in the CTCF-cKO mice. I also found that the CTCF deficiency lead to morphological changes of dendrite and spine formation during neuronal maturation. These results indicate that CTCF is required for the refinement of somatosensory maps and stochastic expression of clustered Pcdh genes in neurons.
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Research Products
(10 results)
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[Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered Protocadherin genes, generating single neuron diversity2014
Author(s)
Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Makoto S, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T
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Journal Title
Neuron.
Volume: 82
Pages: 94-108
DOI
Peer Reviewed
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