2013 Fiscal Year Final Research Report
Surface properties of DNA brush layers and their applications
| Project/Area Number |
24700488
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KANAYAMA Naoki 独立行政法人理化学研究所, 前田バイオ工学研究室, 研究員 (80377811)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | DNA / 界面 |
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| Research Abstract |
In general, double-stranded DNA (dsDNA) undergoes the small thermal fluctuations that transiently break hydrogen bonds and unstuck bases (so-called "breathing") even below the melting temperature. A base pair that is located at the terminal exhibits a higher frequency of breathing than those in the middle part of dsDNA strand. Such conformational dynamics of dsDNA play important roles in various biological processes, including DNA transcription and replication. In this study, we found that the surface properties of a brush layer composed of dsDNA largely depend on their terminal dynamics.
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