2013 Fiscal Year Final Research Report
Microbial analysis of subglacial samples drilling at Dome Fuji, Antarctica
| Project/Area Number |
24710020
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ |
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Principal Investigator |
SEGAWA Takahiro 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, 助教 (90425835)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アイスコア / 微生物 / 極限環境 / 古代試料 |
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| Research Abstract |
The ice cores from Antarctica could preserve microorganisms in the ancient atmosphere trapped in the snow and ice from the ice sheet. Subglacial environment are thought to be contained ancient ecosystems. We examine microorganisms in the ice core samples near the bedrock by molecular DNA techniques. We extracted the inner part of ice core samples using the melting device special designed for biological analysis. Small DNA fragments were extracted from ice samples and sequenced using Illumina Hiseq and Miseq sequencers. We carried out taxonomic and functional binning of the metagenomic DNA, and attempted to reconstruct the organisms. Our data support that microorganism have existed in this subglacial environment.
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