2013 Fiscal Year Final Research Report
Development of the structure analytical method for protein having difficulty in crystallization by the solid-state NMR
| Project/Area Number |
24750014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 分子構造 / 固体ＮＭＲ |
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| Research Abstract |
In this study, the distances between two carbon nuclei in amino acids were estimated from time-dependence of signals recorded by measurements of two-dimensional exchange solid-state nuclear magnetic resonance. We analyzed two amino acids (l-Leucine, l-Alanine) which were labeled two carbon nuclei with carbon-13. We succeeded to estimate the distance between the two carbons of methine and methyl groups in l-Alanine from 0.9 to 1.6 angstroms. Although it was estimated that the distance between carbon nuclei of methine and carboxyl groups in l-Leucine was less than 1.6 angstroms, we could not estimate the lower limit of the distance of l-Leucine. From the above, it is indicated that ranges of distances among carbon nuclei directly-bonding hydrogen nuclei are possibly estimated.
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