2014 Fiscal Year Final Research Report
Mechanism for the signal transduction of CO-gas generated by heme oxygenase together with other proteins in the heme metabolism
| Project/Area Number |
24750169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kurume University |
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Principal Investigator |
HARADA Jiro 久留米大学, 医学部, 講師 (10373094)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ヘム酵素 / ヘムオキシゲナーゼ / NMR緩和分散測定 / ヘム代謝 |
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| Outline of Final Research Achievements |
Heme oxygenase catalyzes the degradation of heme. Heme oxygenase has been well characterized and revealed its heme binding mode in detail. In this study, we elucidated dynamics of Heme oxygenase by relaxation dispersion NMR spectroscopy, and detected structural fluctuation of a loop between C- and D-helices located on the surface. Thus far, the loop region has not been paid attention to, because it is located on the protein surface and far from the heme-binding site. Hence, we analyzed and characterized Leu77 and Phe79 which were the fluctuated amino acid residues in the loop.
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| Free Research Field |
分子生物学/生化学
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