Development of novel culture technique for neural stem cells using polyacrylic acid-grafted fabrics

2014 Fiscal Year Final Research Report

• Project/Area Number: 24760652
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka Prefecture University
• Principal Investigator: MORI Hideki 大阪府立大学, 理学(系)研究科(研究院), 助教 (30450894)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: neural stem cell / Nylon / fabric / cell culture / spheroid

Outline of Final Research Achievements

Acrylic acid (AAc) was grafted onto Nylon (PA66) fabrics through graft polymerization induced by gamma-ray pre-irradiation. Activation of the carboxyl groups in poly AAc (PAAc) induced by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/ N-hydroxyscuccinimide (NHS) treatment allowed covalent immobilization of proteins (Matrigel, etc.). The effects of the PAAc-graftred and protein-immobilized fabrics on the adherence and differentiation of mouse neural stem/progenitor cells (NSPCs) were investigated. Matrigel-immobilized PA66 fabric was the best for the growth of NSPCs in the experimental fabrics.

Free Research Field

生物工学、幹細胞生物学