2013 Fiscal Year Final Research Report
Metabolic analysis of a PET degradation bacterium
| Project/Area Number |
24780078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ポリエチレンテレフタレー ト / ゲノム解析 / トランスクリプトーム解析 / リパーゼ / クチナーゼ / クロロゲン酸エステラーゼ |
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| Research Abstract |
In order to elucidate the mechanism that Ideonella sp. No. 201-F6 strain grows on poly(ethylene terephthalate) (PET) as a carbon source, enzymes involved in the PET metabolism were explored. Whole genome analysis of this strain revealed the metabolic potential for various aromatic compounds containing terephthalic acid. Biochemical analyses for selected proteins through the genome/transcriptome information based screening identified two novel enzymes functioning as PET hydrolase and mono(2-hydroxyethyl) terephthalic acid hydrolase, respectively. These discoveries suggested the metabolic route from PET to carbon dioxide and water in this bacterium.
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