2014 Fiscal Year Final Research Report
Identification of essential elements for the plasmid transfer between different bacteria
| Project/Area Number |
24780087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | プラスミド / 接合伝達 / 核様態タンパク質 |
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| Outline of Final Research Achievements |
The objective of this study is to find essential elements for the conjugative transfer of plasmid between different bacteria using pCAR1. Three kinds of nucleotide-associated proteins (NAPs) encoded on pCAR1 (Pmr, Phu, and Pnd) were shown to be important elements for its replication, maintenance and transfer in different host strains. Products of ORF145 and ORF146 on pCAR1 were not essential for the transfer but they may affect the transfer frequency of the plasmid. NAP genes were shown to be distributed on various transmissible plasmids. We successfully developed a method to detect, separate, and analyze ‘transient’ transconjugants, which would be important for the spread of plasmids in different host strains.
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| Free Research Field |
環境微生物学
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