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2013 Fiscal Year Final Research Report

Elucidation of the molecular network controlling sperm acrosome formation in mammals

Research Project

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Project/Area Number 24780327
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KANEMORI Yoshinori  筑波大学, 生命環境系, 助教 (40529088)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsマウス / 精子形成 / 受精
Research Abstract

Mammalian sperm possess a cap-shaped, exocytotic vesicle, acrosome that is located at the anterior part of the nucleus. ACRBP/sp32, is an acrosomal protein, initially identified as a binding protein for the precursor (proACR) of sperm serine protease ACR. Unlike other mammalian ACRBPs, two forms of Acrbp mRNA, wild-type ACRBP-W and variant Acrbp-V5 mRNAs, were generated by alternative splicing of Acrbp in the mouse. In this study, we produced knockout mice lacking ACRBP to uncover the role of ACRBP in spermatogenesis and fertilization. These results demonstrate that ACRBP-V5 palys key role in condensation and subsequent spread of acrosomal proteins in the acrosome during spermiogenesis. It is also suggested that ACRBP-W may function in the retention of proACR in the acrosome until after sperm undergo acrosomal exocytosis.

  • Research Products

    (6 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse2013

    • Author(s)
      Kanemori, Y., Ryu, J. H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
    • Journal Title

      Biol. Reprod.

      Volume: 88 Pages: 1-8

    • Peer Reviewed
  • [Presentation] 精子アクロソームタンパク質ACRBPとACRのダブル欠損マウスの解析2013

    • Author(s)
      高嶋明日登、兼森芳紀、首藤舞、康宇鎮、馬場忠
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(ポスター)
    • Year and Date
      2013-12-04
  • [Presentation] Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis.2013

    • Author(s)
      Kanemori, Y., Sudo, M., Koga, Y., Okabe, M., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization & Activation of Development
    • Place of Presentation
      Holderness School Holderness, NH, USA (ポスター発表)
    • Year and Date
      2013-07-15
  • [Presentation] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis2013

    • Author(s)
      Sudo, M., Kanemori, Y., Ryu, JH., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, SI., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization & Activation of Development
    • Place of Presentation
      Holderness School Holderness, NH, USA (ポスター発表)
    • Year and Date
      2013-07-15
  • [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization2012

    • Author(s)
      柳辰協、首藤舞、兼森芳紀、柏原真一、馬場忠
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(ポスター発表)
    • Year and Date
      2012-12-14
  • [Remarks]

    • URL

      http://www.acroman.org/

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Published: 2015-06-25  

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