2013 Fiscal Year Final Research Report
Investigation of Baf53a and Oct3/4 in the self-renewal of ES cells
| Project/Area Number |
24790274
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Baf53a / Oct3/4 / クロマチン・リモデリング |
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| Research Abstract |
Several transcription factors are involved in the self-renewal of ES cells. In the present study, I examined relationships between Oct3/4 (transcription factor) and Baf53a (chromatin remodeling factor). Baf53a mRNA expressed in the undifferentiated ES cells, and decreased after Oct3/4 repression. Also, Baf53a associated with the POU domain of Oct3/4. Loss of function experiment of Baf53a by RNAi revealed that Baf53a was involved in proliferation and acetylation of histon H4. Taken together, these results suggest that Baf53a, together with Oct3/4, is involved in proliferation and epigenomic maintenance of ES cells.
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