A study on energy metabolism of fox tapeworm Echinococcus multilocularis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790407
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nihon University
• Principal Investigator: MATSUMOTO Jun 日本大学, 生物資源科学部, 准教授 (70296169)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 寄生虫 / エキノコックス / エネルギー代謝 / メタボロミクス / グルコース / トランスポーター / 創薬 / 寄生適応

Research Abstract

Echinococcus multilocularis (Em) is a zoonotic parasite, distributed widely in northern hemisphere including Japan. The larval stage of Em causes life-threatening disease in humans called alveolar echinococcosis. The present study aimed to provide an overview of energetic metabolism pathway in Em. The result of metabolomics analysis using larval Em cultured under aerobic/anaerobic condition suggests that the parasite possesses both aerobic and anaerobic metabolic pathways. The presence of the putative anaerobic pathway is consistent with our previous study (Matsumoto et al., 2008), leading us to infer that the parasite uses this unique metabolic pathway to survive in anaerobic conditions in host tissues. In addition, we obtained the complete sequence for 3 glucose transporter genes, which are supposed to be involved in energy uptake by Em. Further analyses on energy metabolism in Em are presently carried out to identify novel targets for chemotherapy of alveolar echinococcosis.

Research Products

• [Journal Article] A dog of Echinostoma hortense infection with enteritis diagnosed by upper gastrointestinal endoscopy (2013)
  • Author(s): Okanishi, H., Matsumoto, J., Nogami, S., Kagawa, Y., and Watari, T
  • Journal Title: Journal of Veterinary Medical Science Volume: 75(7) Pages: 991-994
  • Peer Reviewed
• [Journal Article] Characterization of a surface glycoprotein from Echinococcus multilocularis and its mucosal vaccine potential in dogs (2013)
  • Author(s): Kouguchi, H., Matsumoto, J., Nakao, R., Yamano, K., Oku, Y., and Yagi, K
  • Journal Title: PLoS ONE Volume: 8(7) Pages: e69821
  • Peer Reviewed
• [Journal Article] Successful resolution of esophageal granulomas in a dog infected with Spirocerca lupi (2013)
  • Author(s): Okanishi, H., Matsumoto, J., Aoki, H., Kagawa, Y., Asano, K., Nogami, S., and Watari, T
  • Journal Title: Journal of Veterinary Medical Science Volume: 75(12) Pages: 1629-1632
  • Peer Reviewed
• [Journal Article] Case report : First confirmed case of canine peritoneal larval cestodiasis caused by Mesocestoides vogae (syn. M. corti) in Japan (2013)
  • Author(s): Kashiide, T., Matsumoto, J., Yamaya, Y., Uwasawa, A., Miyoshi, A., Yamada, K., Watari, T., and Nogami, S.
  • Journal Title: Veterinary Parasitology Volume: 201(1-2) Pages: 154-157
  • Peer Reviewed
• [Journal Article] 寄生虫に学ぶOne Health : エキノコックスを例に (2013)
  • Author(s): 松本淳
  • Journal Title: Twig's(日本大学獣医学会誌) Volume: 59 Pages: 1-10
• [Presentation] Echinococcus multilocularis (larval stage)ミトコンドリアのフマル酸呼吸を薬剤標的とした新規薬剤開発 (2014)
  • Author(s): 遠海重裕、大森惇子、二橋望、稲岡健ダニエル、坂元君年、松本淳、孝口裕一、八木欣平、片倉賢、奥祐三郎、藤田修、野崎智義、斎本博之、北潔
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス(松山).
  • Year and Date: 2014-03-27
• [Presentation] エキノコックス由来表面糖タンパク質の性質とそのイヌにおける潜在的粘膜ワクチン効果 (2013)
  • Author(s): 孝口裕一、松本淳、中尾亮、山野公明、奥祐三郎、入江隆夫、八木欣平
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜パシフィコ(横浜)
  • Year and Date: 2013-09-13
• [Book] 獣医公衆衛生学I (2014)
  • Author(s): 松本淳(分担)
  • Total Pages: 186-188
  • Publisher: 文永堂出版
• [Book] 獣医公衆衛生学II、pp.152-157. (2014)
  • Author(s): 松本淳(分担)
  • Publisher: 文永堂出版
• [Book] 寄生虫病学
  • Author(s): 松本淳(分担)
  • Publisher: 緑書房(印刷中)