2013 Fiscal Year Final Research Report
Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region
| Project/Area Number |
24790650
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
WATANABE Ken 科学警察研究所, 法科学第一部, 研究員 (20532047)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 物体検査 / 人体液斑の同定 / DNAメチル化 |
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| Research Abstract |
In this study, we analyzed aged stains of various body fluids by bisulfite sequence analysis for the methylation status in the previously reported semen-specific hypomethylated region, DACT1. The results indiate that the methylation status is stable in each old stain and that this can be a useful marker for forensic semen identification. We further developed a TaqMan realtime PCR detection method to make it easier to analyze the methylation status of DACT1 region. This method can be a useful supplementary tool for semen identification, especially from aged stains.
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