2014 Fiscal Year Final Research Report
Effect of fetal membrane-derived mesenchymal stem cells in rats with acute pancreatitis
| Project/Area Number |
24790670
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Research Collaborator |
KAWAKUBO Kazumichi 北海道大学, 大学院医学研究科, 助教 (80633578)
OHNISHI Shunsuke 北海道大学, 北海道大学病院, 講師 (10443475)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 間葉系幹細胞 / 膵炎 / 卵膜 |
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| Outline of Final Research Achievements |
Objectives: We investigated the effect of rat FM-derived mesenchymal stem cells (FM-MSCs) and human amnion-derived MSCs (AMSCs) on the therapeutic effects in rats with acute and chronic pancreatitis.
Methods: Acute pancreatitis was induced by intraductal injection of taurocholate, and rat FM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and human AMSCs were transplanted intravenously.
Results: Transplantation of rat FM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. Human AMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.
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| Free Research Field |
消化器内科学
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