Induction of high quality induced hepatocytes utilizing the human artificial chromosome

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790696
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: SUZUKI Teruhiko 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (70621027)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 染色体 / ヒト人工染色体 / リプログラミング

Outline of Final Research Achievements

To enable highly efficient loading of multiple gene expression units to the human artificial chromosome (HAC), I developed Simultaneous/sequential Integration of Multivector system (SIM system). This system allows for simultaneous integration of maximum three gene-loading vectors carrying genes of interest by co-transfecting recombinase/integrase expression vectors. This system is useful to construct the HACs for cell reprogramming and monitoring of cell differentiation.

Free Research Field

染色体工学