The Signaling Pathway of Lysophosphatidic Acid in Head and Neck Squamous Cell Carcinoma:The Role of Non-EDG Family Receptors

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791792
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: University of the Ryukyus
• Principal Investigator: MATAYOSHI Sen 琉球大学, 医学部附属病院, 助教 (60448587)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リゾフォスファチジン酸 / LPA / 頭頸部癌 / Gタンパク共役受容体

Outline of Final Research Achievements

Lysophosphatidic acids (LPAs) have been implicated in the tumorigenesis of head and neck squamous cell carcinoma (HNSCC). LPA receptors belong to endothelial differentiation gene (Edg) family (LPA1-3) and phylogenetically distant non-Edg family (LPA4- 6). In the present study, we investigated the effect of ectopic expression of LPA4 in SQ-20B, a HNSCC cell line, which expressed trivial level of endogenous LPA4. LPA stimulated proliferation of SQ-20B cells. Infection with doxycycline-regulatable adenovirus vector expressing green fluorescent protein-tagged LPA4 (AdvLPA4G) abolished LPA-stimulated proliferation in SQ-20B cells with the accumulation of G2/M-phasic cells. In the presence of Ki16425 or Rac1 inhibitor, ectopic LPA4-expressing SQ-20B cells showed further down-regulation of the proliferation. LPA induced cell motility was suppressed by ectopic LPA4 expression. Our data suggest that LPA4 signaling potentially modulates malignant behaviors of SQ-20B cells.

Free Research Field

耳鼻咽喉科学