2015 Fiscal Year Annual Research Report
神経芽細胞腫予後良好３因子の分化機序の解明と治療法開発
| Project/Area Number |
24791889
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| Research Institution | Hiroshima University |
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Principal Investigator |
山岡 絵美 (福田絵美) 広島大学, 自然科学研究支援開発センター, 研究員 (20403503)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | Neuroblastoma |
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| Outline of Annual Research Achievements |
Neuroblastoma (NB) is the most common cancer in infants arising from neural crest, is classified unfavorable and favorable outcome. Unfavorable group of neuroblastoma is increasing rapidly, whereas favorable group differentiate or regression.
Recently, we assessed gene expression analysis between favorable group and unfavorable group of neuroblastoma by oligo-microarray then extracted the top three candidate genes including DHRS3, NR0B1, and CYP26A in the favorable groups and performed functional analysis.
Established knockdown and overexpression neuroblastoma stable clones in TGW, SK-N-SH, NH12 caused cell growth inhibition, suppression of colony formation ability. We also observed that the overexpression of DHRS3 promotes lipid droplet accumulation and that involved in cell differentiation. Further RNA-seq and IPA pathway analysis revealed that these genes related to the network of lipid metabolism, molecular transport, neurological diseases etc.
So we suppose that DHRS3, NR0B1, and CYP26A1 may be the growth suppressor in tumor neuroblastoma cell lines.
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