2015 Fiscal Year Final Research Report
Tooth and periodontal tissue regeneration with purified mesenchymal stem cells and gene transfer technique
| Project/Area Number |
24792151
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tohoku University (2014-2015)
Keio University (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 間葉系幹細胞 / 歯胚再生 / 再生医療 |
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| Outline of Final Research Achievements |
The cultivable mesenchymal stem cell (MSC) has self-renewal competence. However, self-renewal ability of MSC shows a decrease after several passages using adhesion culture system. In addition, differentiation advancing and cell phenotype changing also appear in MSC after long term culture using the conventional adhesion culture. It is necessary to multiply the purified MSC in vitro without losing their stem-ness for their use in tooth germ reproduction. Therefore, establish a new culture method to maintain the stem-ness of the MSC is imperative.
Currently applicant to maintain the undifferentiated MSCs in a particular environment. Further, this culture method could be resurrected undifferentiated stage. This method can maintain the stem-ness of MSC through forming a three-dimensional cell mass. This research is delayed from the plan, but now, it is carried out to continue the research for the realization of the tooth germ and periodontal tissue regeneration using this cell mass.
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| Free Research Field |
再生歯科
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