2013 Fiscal Year Final Research Report

Elucidation of mechanism of action of novel antimicrobial peptides from rice and purification and identification of their target compounds in cells.

Research Project

Project/Area Number	24860030
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Biofunction/Bioprocess
Research Institution	Niigata University
Principal Investigator	ISHIYAMA Yohei 新潟大学, 産学地域連携推進機構, 研究員 (50634854)
Project Period (FY)	2012-08-31 – 2014-03-31
Keywords	抗菌ペプチド / 抗生物質 / 無細胞タンパク質合成 / 微生物 / 作用機構
Research Abstract	The purpose of this study was to reveal modes of action of antimicrobial peptides in cells. Several methods using cell-free protein synthesis system were developed for achieving this purpose. By using our methods, we could confirm the known modes of action of conventional antibiotics, such as streptomycin, fosfomycin and BLEOCIN. We were also able to not only assess the unknown abilities of antimicrobial peptides to inhibit protein synthesis, but also elucidate the inhibitory steps in protein synthesis process, including transcription, translation and refolding. Moreover, we found that a purification method using antimicrobial peptide-immobilized magnetic beads was effective to isolate target compounds in cells.