Investigation on coordinate regulation of reproduction and related behaviors by kisspeptin neuronal system

2013 Fiscal Year Final Research Report

• Project/Area Number: 24870007
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokyo
• Principal Investigator: KANDA Shinji 東京大学, 理学(系)研究科(研究院), 助教 (50634284)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: キスペプチン / in situ hybridization / パッチクランプ / トランスジェニック / ノックアウト

Research Abstract

In this study, to unveil the novel neuronal circuits that are regulated by kisspeptin neurons in vertebrates, I visualized kisspeptin receptor-expressing neurons with GFP, and performed RNAseq of GFP positive neurons in medaka. It was shown that the peptidergic neurons that are suggested to be involved in feeding behavior and homeostasis are expressing kisspeptin receptor. These suggestion were finally confirmed by double labeling of GFP and mRNA of a certain neurotransmitter by in situ hybridization. Following electrophysiological examination showed that kisspepitn synthetic peptide depolarize these kisspeptin receptor expressing neurons. As peptidergic neurons such as isotocin and vasotocin neurons were shown to express kisspeptin receptor, knockout medaka lines of these transmitters by using TALEN technology. Further examination of these neural circuits will be examined by more detailed electrophysiology utilizing the generated knockout and transgenic medaka lines.

Research Products

• [Journal Article] Whole brain-pituitary in vitro preparation of the transgenic medaka (Oryzias latipes) as a tool for analyzing the differential regulatory mechanisms of LH and FSH release (2014)
  • Author(s): Karigo, T., Aikawa, M., Kondo, C., Abe, H., Kanda, S. and Oka, Y.
  • Journal Title: Endocrinology Volume: Vol. 155 Pages: 536-547
  • Peer Reviewed
• [Journal Article] Anatomical distribution of sex steroid hormone receptors in the brain of female medaka (2013)
  • Author(s): Zempo, B., Kanda, S., Okubo, K., Akazome, Y. and Oka, Y.
  • Journal Title: J Comp Neurol Volume: Vol. 521 Pages: 1760-1780
  • Peer Reviewed
• [Journal Article] Neuroanatomical evidence that kisspeptin directly regulates isotocin and vasotocin neurons (2013)
  • Author(s): Kanda, S., Akazome, Y., Mitani, Y., Okubo, K. and Oka, Y.
  • Journal Title: PLoS One Volume: Vol.8 Pages: e62776
  • Peer Reviewed
• [Presentation] 真骨魚類を用いた内分泌研究 (2014)
  • Author(s): 神田真司
  • Organizer: 京都大学研究大学強化促進事業百家争鳴プログラム「ムシVSサカナ」
  • Place of Presentation: 京都大学
  • Year and Date: 2014-03-15
  • Invited
• [Presentation] 遺伝子組み換え動物を活用したキスペプチン神経系の隠された機能の探索 (2014)
  • Author(s): 神田真司
  • Organizer: 富山大学大学院理工学部研究部テニュアトラック制度シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2014-03-04
  • Invited
• [Presentation] 脊椎動物キスペプチン神経系の進化と多様な機能 (2013)
  • Author(s): 神田真司,岡良隆
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130327-29
  • Invited
• [Presentation] 性ステロイド感受性を示すKiss1ニューロンの電気生理学的解析 (2013)
  • Author(s): 長谷部政治,神田真司,島田洋之,岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-27
• [Presentation] RFRPニューロンの自発活動とGnRH1ニューロン発火抑制に関する電気生理学的解析 (2013)
  • Author(s): 貝瀬峻,神田真司,岡良隆
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-27
• [Presentation] Functional and evolutionary diversity of vertebrate kisspeptin neuron systems (2013)
  • Author(s): Kanda, S.
  • Organizer: Seminar in NIH
  • Place of Presentation: Rockville
  • Year and Date: 2013-06-26
  • Invited
• [Presentation] 脊椎動物キスペプチン神経系の進化と未知の機能 (2013)
  • Author(s): 神田真司,岡良隆
  • Organizer: 東京大学大気海洋研究所共同利用研究集会
  • Place of Presentation: 柏
  • Year and Date: 2013-06-22
  • Invited
• [Presentation] 終神経ニューロン特異的なgnrh3遺伝子機能阻害 (2013)
  • Author(s): 小島瑠花,高橋晶子,神田真司,岡良隆
  • Organizer: 日本動物学会関東支部第65回大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-16
• [Presentation] 遺伝子改変メダカを用いた黄体形成ホルモン(LH)放出の中枢制御機構の解析 (2013)
  • Author(s): 近藤千香,苅郷友美,相川雅人,神田真司,赤染康久,大久保範聡,阿部秀樹,岡良隆
  • Organizer: 日本動物学会関東支部第65回大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-16
• [Presentation] キンギョ性フェロモン17,20β?Pによる黄体形成ホルモン(LH)分泌中枢制御系の形態学的解析 (2013)
  • Author(s): 吉村充史,河合喬文,善方文太郎,赤染康久,神田真司,岡良隆
  • Organizer: 日本動物学会関東支部第65回大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-16
• [Presentation] Steroid sensitive hypothalamic Kiss1 neurons in medaka do not co-express NKB or DYN (2012)
  • Author(s): Zempo, B., Kanda, S., Akazome, Y., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Generation of kiss1 knockout medaka using efficient new genetic tools, TALENs (2012)
  • Author(s): Takahashi, A., Kanda, S., Akazome, Y., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Electrophysiological and morphological analysis of Kiss1 neurons in trasgenic medaka, Oryzias lapes (2012)
  • Author(s): Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Embryonic expression and function of kisspeptins and their receptors in a teleost fish, medaka (2012)
  • Author(s): Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Welzien, F.A.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Inhibitory regulation of gnrh3 neurons by rfrp neurons in medaka (2012)
  • Author(s): Nakane, R., Kanda, S., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] Effects of kisspeptins on LH secretion and GnRH1 neuronal activities in goldfish and medaka (2012)
  • Author(s): Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo
  • Year and Date: 20121106-09
• [Presentation] gpr54-1-EGFP transgenic medaka - a new approach towards the understanding of novel functions of kisspeptin neurons (2012)
  • Author(s): Kanda, S., Nakajo, M., Lee, J., Oka, Y.
  • Organizer: 2nd world congress of kisspeptin
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20121106-09
• [Presentation] Direct regulation of kisspeptin system on vasotocin and isotocin neurons (2012)
  • Author(s): Kanda, S., Oka, Y.
  • Organizer: Society for Neuroscience
  • Place of Presentation: New Orleans, LA
  • Year and Date: 20121013-17
• [Presentation] 絶食によるメダカ視床下部NPYニューロンのNPY mRNA発現変動 (2012)
  • Author(s): 北原翔一,神田真司,岡良隆
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 20120913-15
• [Presentation] キスペプチン受容体発現ニューロンのGFP標識による未知のキスペプチン機能の探索 (2012)
  • Author(s): 神田真司,李惇馥,岡良隆
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 20120913-15
• [Book] Structure, Synthesis, and Phylogeny of Kisspeptin and its Receptor. In Kisspeptin Signaling in Reproductive Biology (eds K. Alexander and S. Jeremy) (2013)
  • Author(s): Kanda, S. and Oka, Y.
  • Total Pages: 9-26
  • Publisher: Springer Science
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/kanda/