Molecular dissection of post-transcriptional gene regulation by the "mRNA poly(A) tail regulatory system"

2016 Fiscal Year Final Research Report

• Project/Area Number: 25291004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Nagoya City University
• Principal Investigator: Hoshino Shin-ichi 名古屋市立大学, 薬学研究科(研究院), 教授 (40219168)
• Co-Investigator(Kenkyū-buntansha): 細田 直 名古屋市立大学, 薬学研究科(研究院), 講師 (40438198)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: mRNAポリA鎖制御

Outline of Final Research Achievements

The mRNA 3’ poly(A) tail length regulation plays central roles in the post-transcriptional control of gene expression. In this study, we aimed to verify novel regulatory mechanisms of gene expression targeting mRNA poly(A) tail and unraveled the whole picture of the following mechanisms: (i) negative regulation of gene expression by the shortening of the poly(A) tail of mRNA (e.g., c-myc and GluR2), (ii) positive regulation of gene expression by the lengthening of the poly(A) tail of mRNA (e.g., p27kip1, hnRNPA1 and b-catenin), and (iii) stress-induced global regulation of gene expression by the stabilization of mRNA poly(A) tail and formation of stress granules.

Free Research Field

分子生物学