Structural basis for multidrug tripartite efflux pump through both the inner and outer membranes in Pseudomonas aeruginosa.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Yamashita Eiki 大阪大学, たんぱく質研究所, 助教 (00294132)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA ATSUSHI 大阪大学, 蛋白質研究所, 教授 (20188890)
• Research Collaborator: YONEHARA RYO 大阪大学, 蛋白質研究所, 研究員 ; IKEDA ETSUKO 大阪大学, 蛋白質研究所, 博士研究員 ; 堤 研太 大阪大学, 蛋白質研究所, 博士前期課程 ; HIGA YUKI 大阪大学, 蛋白質研究所, 博士前期課程 ; SUMITA KAZUMA 大阪大学, 蛋白質研究所, 博士前期課程
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: X線結晶構造解析 / 膜タンパク質複合体の物質輸送

Outline of Final Research Achievements

The multidrug resistance of Pseudomonas aeruginosa is mainly attributable to high expression of the tripartite efflux pumps that transport antibiotics from within to the external environment. The tripartite efflux pumps, each of which is composed of an inner membrane transporter, a periplasmic adaptor protein and an outer membrane factor, are membrane-protein super-complexes spanning both the inner and outer membranes. In this study, we determined the stoichiometry of the tripartite efflux pump by size exclusion chromatography and gel electrophoresis and the structure of it by negative stain electron microscope. The structures of two major outer membrane factors from multidrug-resistance Pseudomonas aeruginosa were determined by X-ray crystallographic analysis.

Free Research Field

放射光構造生物学