Determination of double-stranded RNA digestion activity in Capsicum and its role

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Kyoto University
• Principal Investigator: Hosokawa Munetaka 京都大学, (連合)農学研究科(研究院), 准教授 (40301246)
• Co-Investigator(Kenkyū-buntansha): AWANO Tatsuya 京都大学, 農学研究科, 助教 (40324660)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トウガラシ / RNase / BC1 / ウイロイド / ウイルス / 二本鎖RNA分解

Outline of Final Research Achievements

Two cultivars (Capsicum annuum), from which RNase activities were not detected. So, we produced F1 hybrid and BC1 lines using the non-RNase cultivars and a cultivar which has very strong RNase activities (C. chinense ‘CP’). The RNase activity analysis using electrophoresis revealed that the gene controlling RNase activities would be single locus. Next, we tried to determine the causal protein of RNase activities using purified proteins, however we could not determine. The best way to determine the causal gene was considered to be positional cloning technique using the BC1 lines.

Free Research Field

蔬菜花卉園芸学