Selection and functionality of candidate strains for probiotics in terms of co-evolution with host livestock

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292165
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Ushida Kazunari 京都府立大学, 生命環境科学研究科(系), 教授 (50183017)
• Co-Investigator(Kenkyū-buntansha): 大熊 盛也 国立研究開発法人理化学研究所, その他部局等, その他 (10270597) ; 丸山 史人 京都大学, 医学(系)研究科(研究院), 准教授 (30423122)
• Co-Investigator(Renkei-kenkyūsha): TSUKAHARA Takamitsu 京都府立大学, 大学院生命環境科学研究科, 特任講師 (90562091) ; INOUE Ryo 京都府立大学, 大学院生命環境科学研究科, 講師 (70443926)
• Research Collaborator: TSUCHIDA Sayaka 京都府立大学, 大学院生命環境科学研究科, 特任講師 (40734687)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 共進化 / 腸内細菌 / ブタ / イノシシ

Outline of Final Research Achievements

Probiotics for livestock become popular, but most of products were derived from human-oriented products without verification in livestock. We isolated intestinal bacteria, which co-evolved with host pig during evolution of Suidae.We collected fresh feces from various animals of Suidae: domestic pigs (Japan and Africa), wild boar in Japan in wild or captivity, Red-river hogs and Worthogs in wild or captivity. NGS-metagenome was done to identify dietary- and host-effect. Bifidobacterium thermascidophilum and Lactobacillus mucosae were identified as Suidae-specific lactic acid bacteria and L. amylovorus is one became predominant with cereal feeding. Among co-evolved lactic acid bacteria, B. thermacidophilum often acquired tetracycline resistance, suggesting inappropriateness of Bifidobacterium as probiotics in pigs. L. mucosae is a better candidate of probiotics for pigs; one strain showed anti-pathogenic activity.

Free Research Field

動物生理学