Development of a gene expression control system using genome editing and its application to insect control

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292202
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: SEZUTSU HIDEKI 国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)
• Co-Investigator(Kenkyū-buntansha): KOMOTO NATUO 国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30355747) ; TOMITA SYUICHIRO 国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30360457) ; HATAKEYAMA MASATSUGU 国立研究開発法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (50281142) ; NIIMI TERUYUKI 名古屋大学, 大学院生命農学研究科, 助教 (00293712)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トランスジェニック昆虫 / ゲノム編集昆虫 / 遺伝子組換え昆虫 / ゲノムエディティング

Outline of Final Research Achievements

To develop a gene expression control system using genome editing for the insect control, we had tried to establish the genome editing system in the various species of insect order Lepidoptera, Diptera, Hymenoptera, and Coleoptera. Using silkworm, Bombyx mori, we have successfully established many gene-knockout strains and studied the gene functions. In addition, we tried to develop a conditional genome editing methods using GAL4/UAS binary system or Tet-On/Off system with a specific promoter. We have also succeeded in gene knockout of a transgene by transcription activator-like effector nucleases (TALENs) in the sawfly, Athalia rosae (Hymenoptera) and the ladybird beetle, Harmonia axyridis (Coleoptera). The results suggested that the genome editing method is available among the various non-model insects.

Free Research Field

昆虫生物学