Determination of PKC substrate involved in neurodegenerative diseases and its application for drug design

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Kobe University
• Principal Investigator: SAITO NAOAKI 神戸大学, バイオシグナル研究センター, 教授 (60178499)
• Co-Investigator(Kenkyū-buntansha): ADACHI Naoko 神戸大学, バイオシグナル研究センター, 助教 (70604510) ; UEYAMA Takehiko 神戸大学, バイオシグナル研究センター, 准教授 (80346254)
• Co-Investigator(Renkei-kenkyūsha): SHIRAFUJI Toshihiko 神戸大学, バイオシグナル研究センター, 研究員 (30595765)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リン酸化酵素 / パーキンソン病 / モデル動物 / 治療薬 / リン酸化プロテオーム

Outline of Final Research Achievements

We identified substrate proteins for PKCg as a new drug target for Parkinson disease. Nine substrate proteins were identified: Connexin-43, Disk1, MADD, CSPa, Calnexin, Stathmin, bPIX, NogoA, Adducin. Furthermore, 1) Phosphorylation of Ser583, Ser340 of bPIX is involved in DA release, 2) Phosphorylation of Connexin-43, MADD, Calnexin and Adducin is also related to DA release, 3) Phosphorylation of Ser10 of CSPa is important for cell viability.

Free Research Field

神経薬理学