Regulation of epidermal innate immunity by Smurf/Arkadia

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293245
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: Koji Sayama 愛媛大学, 医学(系)研究科(研究院), 教授 (80187286)
• Co-Investigator(Kenkyū-buntansha): Toyama Mikiko 愛媛大学, 大学院医学系研究科, 准教授 (60263935) ; Shiraishi Ken 愛媛大学, 医学部附属病院, 講師 (80710863)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Innate immunity / keratinocyte / epidermis / TGF-beta / Smad

Outline of Final Research Achievements

Function of Smurf and Arkadia was analyzed in keratinocytes. Stimulation with TGF-beta enhanced Smad2/3 phosphorylation and Smad7 expression. While TGF-beta stimulation enhaced Smurf1/2 expression, it did not enhance the expression of Arkadia. The phosphorylation of Smad2/3 and the expression of Smad7 were significantly enhanced by reducing Smurf by using shRNA. On the other hand, suppression of Arkadia by shRNA reduced the phosphorylation of Smad2/3 and the expression of Smad7. Furthermore, suppressive effect of TGF-beta on cell growth and migration was enhanced by knockdown of Smurf.

Free Research Field

皮膚科