Creation of Extracellular Matrices for Duplicating Natural Tissue Structure

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293404
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Kato Koichi 広島大学, 医歯薬保健学研究院, 教授 (50283875)
• Co-Investigator(Kenkyū-buntansha): NAKAJI Tadashi 富山大学, 大学院理工学研究部, 准教授 (10543217) ; TANIMOTO Kotaro 広島大学, 大学院医歯薬保健学研究院, 教授 (20322240) ; HIRATA Isao 広島大学, 大学院医歯薬保健学研究院, 助教 (40346507)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 繊維軟骨 / 硝子軟骨 / 間葉系幹細胞 / 組織工学 / 再生医療

Outline of Final Research Achievements

The temporomandibular joint cartilage is made of two distinct tissues including hyaline cartilage in the basal layer and fibrous cartilage in the surface layer. There are various tissues in which such heterotypic tissue structure plays an essential role in their functions. Recently mesenchymal stem cells have been received much attention due to their potentials to regenerate cartilage tissues in patients with a temporomandibular disorder. However, therapeutic outcome does not meet clinical needs because of a difficulty in creating layered structure with hyaline and fibrous cartilages. Our study demonstrated that hyaline and fibrous cartilage-like tissues can be separately induced from mesenchymal stem cells under appropriate culture conditions and that these tissues can be combined by culturing them in an extracellular cage to produce a hyaline-fibrous composite tissue.

Free Research Field

生体材料学