Development of the method for bone regeneration mimicing distraction osteogenesis for extensive bone defects without distraction devices

2015 Fiscal Year Final Research Report

• Project/Area Number: 25293408
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagoya University
• Principal Investigator: HIBI Hideharu 名古屋大学, 医学(系)研究科(研究院), 教授 (90345885)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Akihito 名古屋大学, 大学院医学系研究科, 准教授 (50244083) ; KURODA Kensuke 名古屋大学, 未来材料・システム研究所, 准教授 (00283408) ; KATAGIRI Wataru 名古屋大学, 大学院医学系研究科, 講師 (10437030) ; HATTORI Hisashi 名古屋大学, 医学部附属病院, 講師 (60332699) ; TSUCHIYA Shuhei 名古屋大学, 医学部附属病院, 助教 (20569785)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 移植・再生医療 / 再生医学

Outline of Final Research Achievements

The components of the conditioned media collected from bone marrow mesenchymal stem cells and dental pulp stem cells were detected and partially quantified. We showed that the conditioned media enhanced cell adhesion ability, cell migration ability, angiogenic potential, anti-inflammatory action and anti-apoptotic effect, and that the conditioned media from hypoxic-cultured cells further enhanced the cell migration ability and the angiogenic potential in vitro. We also showed that the conditioned media promoted vascular network formation and consequent bone regeneration, and that the conditioned media from hypoxic-cultured cells further promoted this process in vivo.

Free Research Field

口腔外科学