Explore the molecular mechanism of cellular differentiation induced by PAMPS gels.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25350548
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Hokkaido University
• Principal Investigator: Semba Shingo 北海道大学, 医学(系)研究科(研究院), 特任助教 (40466496)
• Co-Investigator(Kenkyū-buntansha): YASUDA Kazunori 北海道大学, 医学研究科, 教授 (20166507)
• Co-Investigator(Renkei-kenkyūsha): TSUDA Masumi 北海道大学, 医学研究科, 講師 (30431307)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 再生医工材料 / 生体材料 / ダブルネットワークゲル / ハイドロゲル / 軟骨再生

Outline of Final Research Achievements

It is known that PAMPS gel induces the spontaneous regeneration of damaged cartilage, however the detailed molecular mechanism remains unclear. In this study, we identified integrins as PAMPS-binding proteins and found that culturing on PAMPS gel drastically changed the activation of p38 MAP kinase in chondrocytic murine ATDC5 cell. It was also found that the gel enhanced the gene expression related with the extracellular matrix, and secreted extracellular matrix were accumulated in the gel, which enhanced the chondrogenic differentiation of ATDC5 cells cultured on PAMPS gel. These results suggest that PAMPS gel not only alters gene expression via intracellular signaling molecules, such as integrins and MAP kinases, but also functions as a molecular reservoir to accumulate the factors that promote cartilage differentiation.

Free Research Field

生化学