Development of highly sensitive affinity biosensor with interdigitated microelectrodes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25390050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied materials
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: OHNUKI Hitoshi 東京海洋大学, 学術研究院, 准教授 (60223898)
• Co-Investigator(Renkei-kenkyūsha): ENDO. Hideaki 東京海洋大学, 学術研究院, 教授 (50242326)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 電気化学インピーダンス / バイオセンサ / 免疫センサ

Outline of Final Research Achievements

Affinity biosensor based on electrochemical impedance spectroscopy possesses attractive advantages. But the small signal intensity limits the detection performance. In this study, we examined several attempts to increase the intensity using the interdigitated microelectrodes.
First, we intended to optimize the distribution of electric field on the electrode. The edge lines of interdigitated electrodes were covered with SiO2 layer to locate the electric field on the central area of electrode. However, the sensing performance was not as good as normal ones. The result suggested that a disordered surface may increase the performance. To examine this idea, we employed a mixed SAM. One of the SAM molecule has a short and inactive terminal group, and the domains act as pin-hole defects. It was found that the biosensor contains the 75 % areal pin-holes significantly improved the sensing performance. We proposed a model to increase sensing performance by the pin-holes.

Free Research Field

有機薄膜工学