2015 Fiscal Year Final Research Report
Development of intracellular delivery method of proteins by hetero-dimerized leucine zippers
| Project/Area Number |
25410181
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MICHIUE Hiroyuki 岡山大学, 大学院医歯薬学総合研究科, 助教 (20572499)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | ペプチド / DDS / 細胞 / ロイシンジッパー / 細胞内運搬ペプチド / p53タンパク質 / Nanogタンパク質 / ペプチド核酸 |
|---|
| Outline of Final Research Achievements |
For purpose of treatment of disease, we develop a method for delivering a functional proteins or functional peptides into cells. In this work, we synthesized an autophagy-inducing peptide (Beclin 1) modified with a hetero-dimerization leucine zipper peptide (LzK). We also synthesized a cell-penetrating peptide modified with a pair of LzK (LzE). When these peptides were mixed, Beclin 1 was successfully delivered into the cell, and the peptide induced autophagy.
|
| Free Research Field |
生物有機化学
|
